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. 2021 Jul 29;7:197. doi: 10.1038/s41420-021-00543-8

Fig. 6. TG2 mediates vascular stiffening in aging by a dual mechanism.

Fig. 6

A Pulse-wave velocity (PWV) in young and old Tgm2-C277S and WT littermate mice; age-matched TG2−/− (KO) mice are shown for reference. (n = 10 per group; **p < 0.01, ****p < 0.0001 by one-way ANOVA with Bonferroni post hoc correction). B Representative western blot of decellularized aortae from young and old Tgm2-C277S and WT littermate mice. Total TG2 and GAPDH expression are shown as references (n = 5 per cohort). C Tensile testing of aortae from young and old WT (i), Tgm2-C277S (iii), Bl6/129S WT (ii), and TG2−/− (KO) (iv) mice. Data are shown as mean (solid line) ± standard deviation (dotted lines). (n = 8–12 mice per group; ****p < 0.0001 by two-way ANOVA with Bonferroni post hoc analysis). Bar graphs show Incremental elastic modulus (Einc) at a strain of 0.5 (v), and at a strain of 1.8 (vi). (n = 8–12 mice, two samples per mouse; *p < 0.05, ****p < 0.0001 by ordinary one-way ANOVA with Bonferroni post hoc analysis). D Contraction response of aortic segments in response to increasing concentrations of phenylephrine in WT (i), Tgm2-C277S (ii), Bl6/129S (iii), and TG2−/− (iv) (n = 8 mice per group; **p < 0.01 vs. young at same concentration by two-way ANOVA). E Endothelium-dependent relaxation of phenylephrine-preconstricted rings in response to increasing concentrations of acetylcholine in WT (i), Tgm2-C277S (ii), Bl6/129S (iii), and TG2−/− (iv) (n = 8 mice per group; **p < 0.01 vs. young at same concentration by two-way ANOVA). F Endothelium-independent relaxation of phenylephrine-preconstricted aortic rings in response to increasing concentrations of sodium nitroprusside (SNP) in WT (i), Tgm2-C277S (ii), Bl6/129S (iii), and TG2−/− (iv).