Fig. 1. Components of the reb gene cluster are found in diverse pseudomonads.

a Phylogenetic tree of proteins from 38 bacterial strains that are homologous to C. taeniospiralis RebB. The bacterial strains included are those with complete genomes from Fig. 6 of Raymann et al.¹⁹ plus 14 representative pseudomonads (see Supplementary Fig. 1 for the complete annotated tree). C. taeniospiralis RebA and RebB are shown in purple. The cluster-containing C. taeniospiralis RebD and its Pseudomonas homologs is shown in orange. We have assigned the designation “RebP” to Reb homologs that cluster with P. aeruginosa PA14_27630 and PA14_27640 (shown in blue). b Chromosomal arrangement of genes associated with R-body production in a set of strains that represent all arrangements found in pseudomonads. Regulatory genes are shaded yellow. Genes shaded blue are homologs of rebP while genes shaded orange are homologous to C. taeniospiralis rebD. rapA (PA14_27680) and its homologs, which may code for a novel R-body component, are shaded green. The asterisk denotes a gene that is annotated in BioCyc (PA14_RS11205)⁵⁷, but not in the Pseudomonas Genome DB¹⁰.