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. 2021 Jul 29;12:4613. doi: 10.1038/s41467-021-24796-0

Fig. 2. R-bodies are produced by Pseudomonas aeruginosa PA14 biofilms.

Fig. 2

a Schematic showing method for preparation of SDS-insoluble material from PA14 biofilms. b Top 20 most abundant proteins detected in the SDS-insoluble, formic acid-solubilized fraction of WT (one replicate) and Δpel (two biological replicates) biofilms by mass spectrometry (MS). c Scanning electron micrograph (SEM) image of R-bodies (false-colored turquoise) in the WT sample prepared as shown in (a). Scale bar is 1 μm. d SEM images, cartoon representations, and dimensions of individual selected R-bodies in fully coiled and fully extended states. Scale bars is 250 nm. e SEM image of a single, partially extended R-body. Scale bar is 400 nm. Panels c–e scanning electron microscopy are representative of 16 fields of view captured from three experiments.