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. 2021 Jul 29;12:4613. doi: 10.1038/s41467-021-24796-0

Fig. 7. Genes in the PA14 reb cluster contribute to translational inhibition and ribosome cleavage during C. elegans infection.

Fig. 7

a Quantification of fluorescence intensity of irg-1::gfp in the images obtained in b using ImageJ/Fiji. p values indicated were calculated using unpaired, two-tailed t-tests. n ≥ 15 biological replicates from four experiments. b Images of C. elegans containing the irg-1::gfp transgene fed P. aeruginosa with the indicated genotypes for 12 h. Each panel shows fluorescence overlayed with Nomarski. Images are representative of ≥15 replicates from four experiments. Scale bar is 100 µm. c H69 cleavage levels (as percentage of the total 26S rRNA) in adult worms exposed to the PA14 wild type (WT) or the indicated mutants. p values indicated were calculated using unpaired, two-tailed t-tests. n = 4 biological replicates from two experiments. Data are presented as mean values ± SD. d Total RNA profile of adult worms exposed for 24 h to the indicated P. aeruginosa strains. Black arrows denote the H69-cleaved rRNA fragments. RFU relative fluorescence units; “M” indicates a 15-nt marker used in the electrophoretic separation system.