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. 2021 Jul 29;11:15467. doi: 10.1038/s41598-021-95055-x

Figure 1.

Figure 1

Detection of different human immunoglobulin classes by SULFO-TAG Protein-A/G. A MSD plate was coated with varying concentrations of human IgG, IgM, or IgA diluted in PBS and incubated it overnight at 4 °C. On the following day, the plate was washed and blocked with PBS-2% BSA. After another wash, the coated immunoglobulins were detected using 0.2 µg/mL SULFO-TAG labeled Protein-A/G diluted in PBS-2% BSA. The plot shows the ratio of the relative luminescence (RLU) signal responses for coated wells relative to uncoated wells.