Fig. 4.

CD147 deficiency increases the abundance of effector CD8⁺ TILs within tumor tissues. A The frequencies of perforin⁺, granzyme B⁺, and PD-1⁺ cells in the total tumor-infiltrating CD8⁺ T-cell population in B16–F10 tumor-bearing Bsg^WT and Bsg^ΔT mice were analyzed with flow cytometry on day 15 after inoculation (10–11 pairs of mice). B The counts of perforin⁺, granzyme B⁺, IFN-γ⁺, TNF-α⁺, and perforin⁺ granzyme B⁺ tumor-infiltrating CD8⁺ T cells per million total intratumoral cells in B16–F10 tumor-bearing Bsg^WT and Bsg^ΔT mice were calculated with flow cytometry on day 15 after inoculation (6–11 pairs of mice). C The frequency of perforin⁺ cells in the PD-1⁺ Tim-3⁺ CD8⁺ TIL population in B16–F10 tumor-bearing Bsg^WT and Bsg^ΔT mice was analyzed with flow cytometry on day 15 after inoculation (seven pairs of mice). Data are representative of two independent experiments and presented as the mean ± STD