Fig. 8.

Correlations between the CD147 expression on CD8⁺ TILs and clinicopathological characteristics of non-small-cell lung cancer (NSCLC) patients. A Representative figures of double-immunofluorescence staining for CD147⁺ CD8⁺ TILs in NSCLC tumor tissue samples with a high or low CD8⁺ TIL density using anti-CD8a (green) and anti-CD147 (red) antibodies. The nuclei were stained with DAPI (blue). B The correlation between the MFI of CD147 staining on CD8⁺ TILs and the CD8⁺ TIL density was determined with linear- regression analysis (n = 117). C Representative images of CD147⁻ CD8⁺, CD147^medium CD8⁺, and CD147^high CD8⁺ TILs. Arrow, a representative CD8⁺ TIL. D The correlation between the frequency of intratumoral CD147^high CD8⁺ TILs and histological grade was determined with Spearman correlation analysis (n = 117). E The overall survival of NSCLC patients with TNM-stage III or IV disease was compared between the group with a high frequency of CD147⁺ CD8⁺ TILs (>24.64%) and the group with a low frequency (≤24.64%) with the Kaplan–Meier method and log-rank test (n = 51)