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. 2021 Jul 29;11:15431. doi: 10.1038/s41598-021-92176-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Graphical representation of the overall systematic study which shown: selection sequence (antigenic proteins) that suitable for phylogenetic analysis, epitope prediction from the goal protein, construction of vaccine, prediction of B cell epitope, feature assessment of target vaccine. Molecular docking of the vaccine with TLR-2 and TLR-4 immune receptor. Molecular dynamic simulations to estimate the stability of docked complexes. Finally, immune stimulation was employed to understand the immune efficiency of the target vaccine.