Table 1.
Selected promiscuous cytotoxic T-lymphocyte epitope.
| Epitopes | Position | HLA class 1 supertypes (Combined score) | MHC Class 1 alleles | IC50 | Antigenicity |
|---|---|---|---|---|---|
| ATDCSDGNY | 211 | A1 (3.5657) | HLA-A*01:01 | 102.93 | 0.7838 |
| HLA-C*12:03 | 6.55 | ||||
| HLA-C*05:01 | 31.89 | ||||
| HLA-C*14:02 | 205.8 | ||||
| HLA-B*15:02 | 82.46 | ||||
| HLA-A*30:02 | 83.84 | ||||
| KLQPLTFLL | 317 |
A2 (1.4454) A3 (0.7613) A24 (0.7629) B8 (0.8034) |
HLA-A*02:01 | 18.79 | 1.14 |
| HLA-A*32:01 | 50.22 | ||||
| HLA-A*02:06 | 70.07 | ||||
| HLA-C*12:03 | 94.02 | ||||
| HLA-C*07:02 | 123.8 | ||||
| LVRSESAAL | 1086 |
B7 (1.5250) B8 (1.2136) B62 (0.9772) |
HLA-C*12:03 | 84.41 | 0.41 |
| HLA-B*07:02 | 134.24 | ||||
| HLA-C*14:02 | 119.57 | ||||
| HLA-A*30:01 | 214.77 | ||||
| HLA-C*03:03 | 241.33 | ||||
| MLKRRDSTY | 696 |
A1 (0.8621) A3 (0.9329) B8 (1.7688) B62 (1.4024) |
HLA-A*30:02 | 117.88 | 0.90 |
| HLA-B*08:01 | 55.78 | ||||
| HLA-B*15:01 | 63.24 | ||||
| HLA-C*12:03 | 6.67 | ||||
| HLA-C*14:02 | 153.33 | ||||
| RRDSTYGPL | 699 |
B27 (1.5815) B39 (1.1004) |
HLA-C*07:02 | 41.60 | 1.42 |
| HLA-C*12:03 | 17.19 | ||||
| HLA-C*14:02 | 66.93 | ||||
| HLA-B*39:01 | 80.43 | ||||
| HLA-B*27:05 | 173.61 | ||||
| LSIPTNFSF | 780 |
B58 (2.112) B62 (1.39) |
HLA-B*58:01 | 19.24 | 1.127 |
| HLA-B*15:01 | 44.15 | ||||
| HLA-A*23:01 | 194.72 | ||||
| HLA-C*03:03 | 49.73 | ||||
| HLA-C*12:03 | 56.52 | ||||
| HLA-B*57:01 | 236.49 | ||||
| HLA-B*35:01 | 156.16 | ||||
| FSFGVTHEY | 786 |
A1 (2.44) A3 (0.8719) A26 (1.4849) B62 (1.5080) B58 (1.71) |
HLA-B*35:01 | 11.92 | 1.79 |
| HLA-B*58:01 | 94.25 | ||||
| HLA-A*30:02 | 131.05 | ||||
| HLA-A*01:01 | 230.96 | ||||
| HLA-C*07:01 | 21.94 | ||||
| HLA-C*12:03 | 9.27 | ||||
| HLA-A*29:02 | 25.22 | ||||
| HLA-B*46:01 | 47.2 | ||||
| HLA-A*68:01 | 93.86 | ||||
| HLA-B*15:01 | 159.59 |
The epitopes listed in the table are non-allergic, non-toxic, and displayed 100% conservancy among target protein sequence (as predicted using the IDEB conservancy tool). Epitopes with half-maximal inhibitory concentration (IC50) values below 250 were considered to bind suitably with respective human leukocyte antigen (HLA) alleles. The VaxiJen v2.0 server was utilized at 0.4 thresholds to predict the antigenicity of the epitopes.