Skip to main content
. 2021 Jul 16;9:698217. doi: 10.3389/fped.2021.698217

Figure 2.

Figure 2

Mechanical ventilation induces a transient improvement of the media wall thickness (MWT) of the pulmonary arteries in the CDH. (A) Photomicrographs of representative histological sections showing pulmonary arteries immunostained for smooth muscle actin (SMA). (B) MWT measurement of pulmonary arteries on immunostained sections showing increased MWT in CDH animals with improvement in ventilated animals at 30 and 60 min and return to levels similar to non-ventilated CDH animals after 90 min of mechanical ventilation (n = 10 arteries measured in four animals per group). Dashed lines refer to the arteries' media wall thickness. CDH, congenital diaphragmatic hernia. Magnification = ×400; bar = 50 μm. C, control; C30, control ventilated for 30 mins; C60, control ventilated for 60 min; C90, control ventilated for 90 min; CDH, congenital diaphragmatic hernia; CDH30, CDH ventilated for 30 min; CDH60, CDH ventilated for 60 min; CDH90, CDH ventilated for 90 min.