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. 2021 Jul 29;4:921. doi: 10.1038/s42003-021-02450-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Two hundred ng of FLAG-tagged FIP200 was transfected with 20 ng of FLAG-tagged RIG-I and 20 ng of pRL-SV40 (Renilla luciferase as an internal control), together with 200 ng of pISRE-Luc or NF-κB-Luc into HEK293 cells. After 48 h, cells were collected, and the ratio of firefly luciferase to Renilla luciferase was calculated to determine the relative reporter activity. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. (*P < 0.05, by paired two-tailed Student’s t test). b RIG-I was transfected with vector or FIP200 into HEK293 cells. After 48 h, RNA was extracted, and real-time PCR for IFNβ, IP10, and RANTES was performed. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. (*P < 0.05, **P < 0.01, by paired two-tailed Student’s t test). c RIG-I was transfected with vector or FIP200 into HEK293 cells. After 48 h, cell lysates were blotted as indicated. d Two hundred ng of FLAG-tagged FIP200 was transfected with 20 ng of 2CARD-FLAG and 20 ng of pRL-SV40, together with 200 ng of pISRE-Luc or NF-κB-Luc into HEK293 cells. After 48 h, cells were collected, and the ratio of firefly luciferase to Renilla luciferase was calculated to determine the relative reporter activity. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. (**P < 0.01, by paired two-tailed Student’s t test). e The 2CARD was transfected with vector or FIP200 into HEK293 cells. After 48 h, RNA was extracted, and real-time PCR for IFNβ, IP10, and RANTES was performed. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. (*P < 0.05, **P < 0.01, by paired two-tailed Student’s t test). f The 2CARD was transfected with vector or FIP200 into HEK293 cells. After 48 h, cell lysates were blotted as indicated.