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. 2021 Jul 29;4:921. doi: 10.1038/s42003-021-02450-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a RIG-I was transfected with vector, FIP200, or ULK1 into HEK293 cells. After 24 h, cells were infected with the VSV carrying a luciferase gene (VSV-Luc) for 16 h. Luciferase activity was measured to determine relative viral infection activity. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. (*P < 0.05, by two-tailed Student’s t test). b Fip200^f/f and Fip200^−/− MEFs were infected with VSV-Luc for 16 h. Luciferase activity was measured to determine relative viral infection activity. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. The P-value was calculated (two-tailed Student’s t test) by comparison with wild-type cells. *P < 0.05. c Wild type and FIP200 knockout RAW 264.7 macrophages were infected with 0.001 MOI of VSV. After the designated hour post-infection (h.p.i), virus titers were determined by TCID₅₀ in Vero cells. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. The P-value was calculated (two-tailed Student’s t test) by comparison with the wild-type cells. *P < 0.05, **P < 0.01. d Fip200^f/f and Fip200^−/− MEFs were infected with the indicated MOI of HCoV OC43. After 24 h, the RNA levels of viral membrane gene were determined by qPCR. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. The P-value was calculated (two-tailed Student’s t test) by comparison with the wild-type cells. *P < 0.05, **P < 0.01. e Fip200^f/f and Fip200^−/− MEFs were infected with 0.1 MOI of HCoV OC43. After the designated day post-infection (d.p.i), virus titers were determined by TCID₅₀ in Vero cells. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments. The P-value was calculated (two-tailed Student’s t test) by comparison with the wild-type cells. *P < 0.05, **P < 0.01. f Fip200^f/f and Fip200^−/− MEFs were infected with the VACV carrying a luciferase gene (VACV-Luc) for 16 h. Luciferase activity was measured to determine relative viral infection activity. All experiments were biologically repeated three times. Data represent means ± s.d. of three independent experiments.