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. 2021 Jul 29;11:15445. doi: 10.1038/s41598-021-94856-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images depicting co-expression of TRPM6 and TRPM7 proteins in human cardiomyocytes. (a) The immunofluorescence of TRPM7 (green) and TRPM6 (red) in the same LA, RA, LV, and RV cardiomyocyte when using conjugated antibodies (the arrowheads indicate the localization of TRPM6 protein in perinuclear area). (b) Quantification of the staining intensity of the immunodetected conjugated antibodies (spotty) and non-conjugated antibodies (smooth) for both proteins in cardiomyocytes from the four chambers of the heart as indicated. *P < 0.001 2 h vs. 12 h, #P < 0.001 right-sided vs. left-sided heart chambers. Other notations are the same as in Fig. 1.