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. 2021 Jul 29;11:15445. doi: 10.1038/s41598-021-94856-4

Figure 4.

Figure 4

Effect on incubating human cardiomyocytes in acidic solution on the immunofluorescence of TRPM6 and TPRM7. (a, b) Quantification of the intensity of fluorescence at pH = 7.4 (open symbols) and at pH = 5.0 (filled symbols) expressed in arbitrary units (a.u.) with and without divalent cations, respectively. *P < 0.001 pH 5.0 vs. pH 7.4. Other notations are the same as in Fig. 1. Extracellular acidification differently changes the fluorescence level of the immunodetected TRPM7 (filled diamonds) and TRPM6 (filled circles) proteins in human cardiomyocytes depending on the presence of divalent cations in the milieu.