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. 2021 Jul 29;11:15445. doi: 10.1038/s41598-021-94856-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TRPM7 and TRPM6 protein levels and RT-qPCR mRNA relative expression levels in human heart tissue homogenates. (a, b) TRPM7 and TRPM6 proteins are increased in the walls of all heart chambers with IHD (filled columns) vs. non-IHD (unfilled columns). A blinded study-design (with the diagnosis unknown to the investigator) was used for the detection of protein concentration in the various samples. Values (mean ± SEM) are in pg/mL and from 3–33 heart tissue homogenates. *P < 0.05 IHD vs. non-IHD, #P < 0.05 right-sided vs. left-sided using ANOVA with LSD test (Least Significant Difference). (c) mRNA relative expression levels of human Trpm6 and Trpm7 genes detected in the four cardiac chambers from explanted human hearts (n = 3, for each). *P < 0.05 Trpm7 vs. Trpm6, #P < 0.05 right-sided vs. left-sided.