Fig. 1.

Abundances of select RBC molecules anti-correlate with rates of hemolysis during storage.

(A) Schematic of the 13 monozygotic twin pairs and the 5 dizygotic twin pairs analyzed in this study.

(B) Red blood cell (RBC) hemolysis (percent of the total RBCs that were hemolyzed) at day 14 of cold storage across the individual donors in the twin study. Raw data from Ref. [3].

(C) Abundance of the protein glutathione synthetase (GSS; arbitrary units, a. u, based on mass spectrometry signal) in RBCs from donors in the twin study. Raw data from Ref. [4].

(D) Schematic depicting the time points at which samples of the stored RBC units were analyzed for hemolysis. Using samples isolated on the day of donation, RBC proteins and metabolites were quantified by mass spectrometry (“day 0” time point).

(E) Scatter plot of RBC hemolysis at day 14 of storage (%) versus the abundance of the protein GSS.

(F) Rank-ordered plot of RBC molecule correlations (ρ) with hemolysis at day 14, highlighting GSS. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)