Fig. 4.

Computer simulation of ¹³C-MFA for the selection of an effective carbon source. (a) Expanded toy model of central carbon metabolism of E.coli. (b) Comparison of 95% confidence intervals of three anaplerotic reactions (v₄, v₅, and v₆) and one non-anaplerotic reaction (v₇) determined via simulation of ¹³C-MFA using distinct carbon sources. Red dotted lines represent metabolic flux levels of answers. [1–¹³C]glucose: 100% [1–¹³C]glucose, [U–¹³C]glucose: [U–¹³C]glucose and non-labeled glucose at 50:50 ratio, Mixture: [U–¹³C]glucose and [1–¹³C]glucose at 50:50 ratio, Parallel: parallel labeling experiment using 100% [1–¹³C]glucose and the mixture of [U–¹³C]glucose and non-labeled glucose at 50:50 ratio. (c) Comparison of 95% confidence intervals of v₄ determined by simulation of ¹³C-MFA using distinct measured metabolites.