CBRA medium
| Reagent | Final concentration | Amount |
|---|---|---|
| cSFDM | n/a | 997 μL |
| CHIR99021 (3 mM) | 3 μM | 1 μL |
| rhBMP4 (10 μg/mL) | 10 ng/mL | 1 μL |
| Retinoic acid (100 μM) | 100 nM | 1 μL |
| Total | n/a | 1 mL |
Store cSFDM supplemented with CHIR99021 and rhBMP4 at 4°C for up to 1 week
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