Table 3.
Sperm characteristics of roosters fed with fatty acid sources with or without vitamin E.
| Treatments1 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Parameters | Days | 1 (CTR group) | 2 (PSO group) | 3 (SFO group) | 4 (CTR+vitE group) | 5 (PSO+vitE group) | 6 (SFO+vitE group) | SEM | P-value |
| Volume (mL) | |||||||||
| 0 | 0.68 | 0.9 | 0.5 | 0.78 | 0.78 | 0.43 | 0.07 | 0.28 | |
| 20 | 0.66 | 0.8 | 0.46 | 0.86 | 0.66 | 0.48 | 0.07 | 0.53 | |
| 40 | 0.54 | 0.88 | 0.52 | 0.47 | 0.52 | 0.44 | 0.06 | 0.27 | |
| 60 | 0.67 | 0.8 | 0.52 | 0.56 | 0.76 | 0.4 | 0.06 | 0.29 | |
| Concentration (1 × 109) | |||||||||
| 0 | 3.63 | 3.46 | 3.49 | 3.89 | 3.86 | 3.75 | 0.06 | 0.17 | |
| 20 | 3.89 b | 3.88 b | 3.97 b | 4.54 a | 3.93 b | 3.70 b | 0.07 | 0.01 | |
| 40 | 3.73 b | 3.74 b | 3.89 b | 3.70 b | 4.76 a | 3.55 b | 0.1 | 0.0007 | |
| 60 | 3.07 c | 3.55 b | 3.22 bc | 2.90 cd | 5.05 a | 2.59 d | 0.16 | <0.0001 | |
| Viability (%) | |||||||||
| 0 | 82.00 a | 81.75 a | 81.00 a | 83.75 a | 76.75 b | 84.50 a | 0.64 | 0.002 | |
| 20 | 82.50 a | 73.25 c | 79.00 ab | 75.00 bc | 78.50 abc | 76.25 bc | 0.86 | 0.02 | |
| 40 | 84.20 a | 81.67 ab | 82.80 a | 83.40 a | 82.25 ab | 78.00 b | 0.63 | 0.06 | |
| 60 | 73.20 b | 68.00 c | 67.50 c | 73.60 b | 85.00 a | 69.33 bc | 1.25 | <0.0001 | |
| Morphology (%) | |||||||||
| 0 | 13 | 16 | 15.33 | 13.25 | 13.75 | 16.25 | 0.51 | 0.25 | |
| 20 | 14.75 | 14.25 | 13.75 | 14.75 | 12.5 | 14.25 | 0.41 | 0.66 | |
| 40 | 16.0 ab | 18.0 a | 13.0 bc | 15.20 ab | 11.50 c | 15.33 ab | 0.54 | 0.002 | |
| 60 | 23.6 | 18.8 | 21.8 | 20.2 | 24.4 | 19.33 | 0.85 | 0.31 | |
| Membrane Integrity (%) | |||||||||
| 0 | 82.60 a | 80.75 ab | 81.67 a | 76.00 b | 84.00 a | 79.25 ab | 0.77 | 0.03 | |
| 20 | 79.00 ab | 73.00 d | 81.25 a | 73.50 d | 75.25 cd | 77.00 bc | 0.69 | 0.0001 | |
| 40 | 77.40 c | 74.33 c | 73.40 cd | 85.60 b | 92.25 a | 68.67 d | 1.6 | <0.0001 | |
| 60 | 70.60 b | 74.60 b | 71.25 b | 71.20 b | 85.00 a | 70.67 b | 1.09 | <0.0001 | |
Data are presented as means ± SEM (n = 5 birds per each treatment).
Sperm Parameters: Morphology (Hancock solution), viability (Eosin-Nigrosin), plasma membrane integrity (Hypo-osmotic swelling test) were assessed.
Abbreviations: PSO, pumpkin seed oil; SFO, sunflower oil; vitE, vitamin E.
a-bDifferent letters within the same row show significant differences among the groups (P < 0.05).
Roosters received 6 types of treatments: 1) basal diet as control group (Control), 2) basal diet supplemented with 2% pumpkin seed oil as n-3 source of fatty acids (PSO group), 3) basal diet supplemented with 2% sunflower oil as n-6 source of fatty acids (SFO group), 4) basal diet supplemented with 200 mg/kg vitamin E (CTRL + vitE group), 5) basal diet supplemented and 2% pumpkin seed oil along with 200 mg/kg vitamin E (PSO + vitE group) and 6) basal diet supplemented with 2% sunflower oil along with 200 mg/kg vitamin E (SFO + vitE group).