FIGURE 1.

Identification of a screened H₂O₂ sensitive mutant S28515. (A) S28515 is more sensitive to 2.5 mM H₂O₂. The wild type and S28515 strains were cultured on complete media (CM) plates or CM containing H₂O₂ (2.5 mM) plates in the dark for 10 days at 25°C and then representative colonies were photographed 7 days post-inoculation (dpi). (B) S28515 is defective in pathogenicity. Conidial suspensions of the wild type and S28515 strains were sprayed on rice seedlings. Diseased leaves were photographed 7 dpi. (C) Southern blot analysis of S28515. The total genomic DNA was digested with EcoRV and XbaI, and probed with the partial HPH fragment. (D) Insertion site analysis. The green arrow indicates the MGG_08005 T-DNA insertion site. The gray area represents the mRNA transcriptional region of the gene MGG_08005. The red rectangle represents PTP/DSP domain. The capital letters ATG and TGA represent the translation start and stop sites, respectively. (E) Sequence alignment. The catalytic domain of MoPTEN was compared with the phosphatase catalytic domains of reported species, which include Magnaporthe oryzae Guy11, Colletotrichum graminicola M1.001, Fusarium graminearum PH-1, Ustilago maydis 521, Botrytis cinerea B05.10, Homo sapiens, Saccharomyces cerevisiae S288C, and Schizosaccharomyces pombe. (F) Functional complementation of MoPTEN for ScTEP1 in S. cerevisiae. A 10 μL droplets containing the indicated concentration of yeast cells were inoculated on to the solid YPD medium plates (20 mM wortmannin added). Wortmannin resistant phenotype of ΔTEP1 was restored to sensitivity by transferring the MoPTEN gene in the yeast. Representative plates were photographed 3 dpi.