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. 2021 Jul 16;12:715773. doi: 10.3389/fmicb.2021.715773

FIGURE 2.

FIGURE 2

Temporal and spatial dynamics of the MoPTEN gene expression in M. oryzae. Green fluorescence signals of the MoPTEN-GFP protein were examined by epifluorescence (GFP) microscopy in different developmental stages of M. oryzae. All the hyphae, conidia, appressoria and invasive hyphae in leaf-sheath cells were transferred to cover slips before green fluorescence signals observation. The 6-day-old hyphae grown on potato dextrose agar (PDA), conidia harvested from the 10-day-old colonies grown on oatmeal-tomato agar (OTA) media, germinated conidia (6 h), appressoria (12 h), invasive hyphae at 12 h post-inoculation (hpi), and invasive hyphae at 48 hpi were used for green fluorescence signals observation on Nikon Eclipse Ni-U microscope (Nikon, Tokyo, Japan). Scale bar = 20 μm.