FIGURE 3.

Identification of two alternative splicing variants of MoPTEN. (A) Sequencing of two transcripts of the MoPTEN gene. The partial sequencing results of the two transcripts were shown. The retained intron is underlined, and the numbered arrows indicate two ends of an exon. (B) Schematic representation of the MoPTEN gene and its splice variants. Alternative splicing occurs in the second intron of MoPTEN. The retention or splicing of the second intron leads to two transcript forms, MoPTEN-1 and MoPTEN-2. The upper number stands for the two ends of an exon. (C) MoPTEN-1/2 expressed in Escherichia coli. Purified both MoPTEN-1/2 were indicated in SDS-PAGE. (D,E) Measurement of lipid and protein phosphatase activity. PIP3 and phosphopeptide were used as substrates for activity measurement, respectively; the denatured recombinant proteins were used as negative control, and the recombinant human PTEN and PTP1B were used as positive controls. Error bars represent ± SD of three independent repeated samples.