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. 2021 Jul 16;12:708645. doi: 10.3389/fphar.2021.708645

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Copyright © 2021 Wang, Wang, Cao, Dong and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of melatonin on MT2/ERK/Nrf2 signaling in the HT-22 cells exposed to Erastin. The HT-22 cells exposed to Erastin and melatonin or Fer-1 were pretreated with 4P-PDOT, PD98059 and ML385, respectively. (A) Relative cell viability (%) (n = 5). (B) Fluorescence staining of ROS. Bar = 200 μm. (C) Relative quantification of intracellular ROS level (n = 5). (D–J) Relative protein levels of p-ERK1/2, Nrf2, GPX4, TFR1, DMT1 and FPN normalized to β-actin (n = 6). (K–L) SOD and MDA levels (n = 6). (M–O) Relative mRNA levels of iron transporter proteins (TFR1, DMT1 and FPN) (n = 6). Differences were assessed using one-way ANOVA. The result represents the mean ± standard error of the mean. Values not sharing a common superscript letter differ significantly at p < 0.05; those with the same letter do not differ significantly (p ≥ 0.05).