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. 2021 Jul 16;9:702890. doi: 10.3389/fcell.2021.702890

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Copyright © 2021 Wang, Zhang, Liu, Xu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased hepatic neutrophils were observed in colitis-induced liver injury. The infiltration and number of hepatic neutrophils were assessed by (A) immunohistochemistry (IHC) for myeloperoxidase (MPO) and (B,C) flow cytometry (anti-neutrophil⁺SSA^high) in the following groups: normal control (NC), acute dextran sulfate sodium (DSS) colitis (aDSS), sinusoidal obstruction syndrome (SOS), DSS + SOS, high-dose ConA hepatitis (H-ConA), DSS + H-ConA, drug-induced liver injury (DILI), DSS + DILI, liver fibrosis (LF), DSS + LF, non-alcoholic steatohepatitis (NASH), and DSS + NASH. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001 by t-test; n = 5–6 in each group; figures are representative of three experiments.