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. 2021 Jul 16;9:702890. doi: 10.3389/fcell.2021.702890

FIGURE 4.

FIGURE 4

Infiltration and accumulation of neutrophils are related with the expression of CXCL1 in various tissues. The number of neutrophils and expression of CXCL1 were evaluated at day 3 in the following seven groups: normal control (NC), acute dextran sulfate sodium colitis (aDSS), sinusoidal obstruction syndrome (SOS), lipopolysaccharide-induced pneumonia (LPSPn), SOS + LPSPn, DSS + SOS, and DSS + SOS + LPSPn. (A) In the peripheral blood, a whole blood cell test was performed and CXCL1 was tested by enzyme-linked immunosorbent assay. (B) In the liver, the number of neutrophils was evaluated by flow cytometry (anti-neutrophil+SSAhigh), and mRNA level of CXCL1 was tested. (C) In the lung, immunohistochemistry for myeloperoxidase (MPO) and flow cytometry were performed and mRNA level of CXCL1 was tested. (D) In the colon, immunohistochemistry for MPO and mRNA level of CXCL1 were tested. Asterisk indicates that the level is significantly different from normal control. *p < 0.05; **p < 0.01; ***p < 0.001 by t-test; n = 5–6 per group; figures are representative of three experiments.