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. 2021 Jul 16;12:696599. doi: 10.3389/fphar.2021.696599

FIGURE 4.

FIGURE 4

Effects of aucuboside on inflammation and apoptosis in colitis. After 13 days of aucuboside treatment, effects on colonic inflammatory mediators (A) COX-2, (B) iNOS, and (C) phosphorylated NF-κB-p65 (p-p65) were studied by western blotting. The effects of aucuboside on apoptosis-related proteins (D) cleaved caspase 3 (cl-caspase3) and (E) Bax were studied by western blotting. (F) TUNEL was used to test the effects of aucuboside on epithelial cell apoptosis in colitis. Data are expressed as mean ± SD. Values in the sham group are 100%, and other data are given relative to the sham group. **p < 0.01 compared with the sham group; ## p < 0.01 compared with the colitis control; n = 6 mice.