Figure 5.

Mitochondrial membrane potential analysis. (A, C) The mitochondrial membrane potential was measured in mutant and control cell lines using the BD Biosciences LSR II flow cytometer and JC-10 Mitochondrial Membrane Potential Assay Kit. The ratios of fluorescence—490/525 nm and 490/525 nm (FL590/FL525)—were recorded to determine the ΔΨm level of each sample. Representative flow cytometry images of mutant cell lines and control cell lines with and without 10 µM of FCCP are shown. (B, D) The geometric means of the FL590/FL525 relative ratios between mutant and control cell lines were calculated to reflect the level of ΔΨm. The averages of three to five determinations for each cell line are shown. Graph details and symbols are explained in Figure 1.