Figure 6.

KLR-70 inhibits the DnaK/DnaJ/GrpE (i.e., K/J/E)-mediated refolding of luciferase. (A) Plot illustrating the normalized luminescence of luciferase (20 nM) refolded in the presence of K/J/E (0.6 μM K, 0.12 μM J and 0.24 μM E) and variable amounts (0 – 400 μM) of KLR-70. The negative control (N.C.) denotes the normalized luminescence arising from luciferase refolding in the absence of K/J/E and KLR-70. (B) Table summarizing the p values pertaining to a comparison of the negative control (N.C.) with luciferase refolding carried out in the presence of K/J/E and variable amounts of the KLR-70 inhibitory peptide. A one-tailed Student T-test assuming unequal variances was performed. Statistical significance was judged using a 95 % confidence interval (p ≤ 0.05). All data represent avg ± std. err. for n = 3 independent experiments