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. 2021 Jul 16;12:696680. doi: 10.3389/fmicb.2021.696680

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Copyright © 2021 Sayed, Abd Elhameed, Abd El-Kareem, Abdel-Malek, Ali, Ibrahim, Sayed, Khalaf, Abdel-Wahid and El-Mokhtar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of the immune transcripts in the PBMCs of AHE patients.(A–F) PBMCs from AHE patients (n = 14) and healthy controls (n = 8) were assessed for the immune transcripts; IFN-Ɣ (A), IL-1β (B), IL-12 (C), IL-2 (D), IL4 (E), and IL-10 (F) by RT-qPCR. The transcript expression for each gene was normalized to the housekeeping gene. Different colors and symbols indicate different patients. Data represented as mean ± SEM. Circle is added around the PBMCs with detectable (+) and (–) RNA strands. *, **, *** indicate that p < 0.05, 0.01, and 0.001, respectively, as determined by Mann–Whitney test.