Figure 5.

LncRNA TUG1 serves as a ceRNA to sponge miR-29c. (A) The putative binding sites between LncRNA TUG1-WT and miR-29c. The sequence of LncRNA TUG1-Mut was also shown. (B) The LncRNA TUG1-WT and LncRNA TUG1-Mut fragments were subcloned into the Renilla luciferase gene pGL3-Luciferase reporter vectors (Promega, USA) to generated pGL3-LncRNATUG1-WT and pGL3-LncRNA TUG1-MUT vectors, respectively. After that, the above vectors were co-transfected with miR-29c mimic or negative control into 293T cells for 24 h, and then the luciferase activities were detected. Data are shown as Mean ± SD and represent three independent experiments. **p < 0.01. NS, no significance.