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. 2021 Jul 16;11:661184. doi: 10.3389/fonc.2021.661184

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Copyright © 2021 Zhu, Li, Wang, Zhou, Fan, Ma, Gao, Gai and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of PTEN expression rescued down-regulate the activity of PI3K/AKT pathway by PCB treatment in MCF-7 and MDA-MB-231 cells. (A) Knockdown of PTEN in MCF-7 and MDA-MB-231 cells was confirmed by western blot analysis. (B) After PTEN protein knockdown, MCF-7 and MDA-MB-231 cells were treatment with PCB (0, 80, 160, or 240 µM) for 72 h and the expression of PI3K, total AKT, phosphorylated AKT were examined. GAPDH served as an internal control. Bands were quantified using Image J software. Each bar represents the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. the control group.