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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: J Allergy Clin Immunol. 2020 May 6;147(1):309–320.e6. doi: 10.1016/j.jaci.2020.04.033

FIG 5.

FIG 5.

Donor chimerism and reconstitution in the spleen of conditioned Rag1mut mice. A, Donor chimerism in T (CD3+), B (CD19+ B220+), and myeloid (CD11b+ B220−) cells was measured by flow cytometry in splenocytes isolated from Rag1-KO (KO) and Rag1-F971L (F971L) mice and shown as proportion of donor CD45.1+ cells and recipient CD45.2+ cells; 1-way ANOVA, Kruskal-Wallis test for multiple comparison on CD45.1+ cell frequencies. B, Splenic T-cell counts are shown. C, Phenotypic analysis results of splenic T lymphocytes were evaluated according to the expression of CD44 in the CD3+CD4+ (left panel) and CD8+ cells (right panel); 1-way ANOVA, Kruskal-Wallis test for multiple comparison on naive T-cell frequencies. D, Splenic B-cell counts are shown. E, B-cell subsets’ distribution in spleen was evaluated as proportion of transitional (Trans), follicular (FO), and MZ B cells, as analyzed by flow cytometry. One-way ANOVA, Kruskal-Wallis test on FO B-cell frequencies. B and D, One-way ANOVA, Kruskal-Wallis test for multiple comparison. A-E, Analyses were performed 16 weeks after the transplant. *P < .05; **P < .005; ***P < .0005; ****P < .0001. Means ± SDs are shown. Eff/mem, Effector/memory.