Figure 5.

Epitope residues of class representative plasma antibodies recognizing three major antigenic sites on EBOV GP. Amino acids and their positions for key binding site residues (green) are indicated for representative base-specific (A), glycan cap-specific (B), or head domain/RBS-specific (C) antibodies and shown on a ribbon diagram of the EBOV GP trimer (PDB ID: 5JQ3) for one protomer. GP1 is in yellow, and GP2 is in red. (D) The NPC1 receptor binding site residues of the RBS (PDB ID: 5F1B) are shown in orange and NPC1 contact region is indicated within a dashed orange shape on GP_CL (top view). RBS residues I113, K114, and G118 identified as critical for binding of head domain/RBS-specific antibodies are shown in green.