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. 2021 Jul 16;12:675534. doi: 10.3389/fimmu.2021.675534

Figure 1.

Figure 1

MSU crystals upregulated the expression of TTP in vitro and in vivo. (A, B) J774A.1 cells were primed with 100 ng/ml LPS for 1 h and then treated with different concentrations of MSU crystals for 12 h. (A) Total RNA was extracted from J774A.1 cells and reverse-transcribed, and the mRNA level of TTP was measured by qRT-PCR. *P < 0.05 vs. without MSU crystals treatment. (B) Total protein was isolated from J774A.1 cells, and TTP protein level was detected by Western blot. *P < 0.05 vs. without MSU crystals treatment. (C, D) J774A.1 cells were primed with 100 ng/ml LPS and then treated with 50 μg/ml MSU crystals at various time points (0, 3, 6, 9, 12 h). (C) Total RNA was extracted from J774A.1 cells, reverse-transcribed, and the mRNA level of TTP was measured by qRT-PCR. *P < 0.05 vs. without MSU crystals treatment. (D) Total protein was isolated from J774A.1 cells, TTP protein level was detected by Western blot. *P < 0.05 vs. without MSU crystals treatment. (E) Immunofluorescence assay was used to detect TTP protein distribution in mouse foot pad tissue sections. Blue shows nuclei stained with Hoechst 33342. Scale bar: 40 μm. (n=4 per group, mean ± SEM), *P < 0.05 vs. without MSU crystals injection in the foot pad tissue. All the data are expressed as the means ± SEM from n=3 independent experiments.