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. 2021 Jul 16;12:675534. doi: 10.3389/fimmu.2021.675534

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Copyright © 2021 lv, Qin, Huang, Jiang, Chen, Long, Ren, Liu, Xie and Zeng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of ATG on MSU crystal-induced inflammatory cell infiltration and cytokine release in a mouse model of peritonitis and arthritis. (A) After the cells precipitated from the peritoneal fluid and were respectively stained with FITC-CD45 Ab, PE-F4/80 Ab, and APC-(Ly-6G and Ly-6C) Ab. The percentage and cell numbers of migrated leukocytes, macrophages, and neutrophils were analyzed using FACS. (B) Paw swelling index. (C) An immunofluorescence assay was used to detect macrophage, neutrophil and MPO positive cells distribution in mouse foot pad tissue sections. Blue shows nuclei stained with Hoechst 33342. (D) Western blot assays were used to measure the protein levels of COX-2 and iNOS in foot pad tissue. n=5 for each group, *P < 0.05 vs. Ctrl; ^#P < 0.05 vs. MSU crystals + vehicle. All the data are expressed as the means ± SEM from n=3 independent experiments.