Figure 1.

Nlrc3^−/− mice are susceptible to HTNV infection. Mice were intraperitoneally inoculated with 5×10⁵ PFUs of HTNV 76-118. (A) Body weights were recorded daily up to 9 dpi for Nlrc3^−/− mice (n=15) and C57BL/6J wild-type (WT) mice (n=10). At the time of inoculation (Day 0), the body weights of mice were set as the baseline, and weights relative to baseline were shown. (B) Body temperature was recorded daily up to day 8 post-infection for Nlrc3^−/− mice (n=25) and C57BL/6J wild-type (WT) mice (n=20). (C) Viral loads in the serum, spleen, and kidney of HTNV-infected mice were measured on 3, 6, 9, and 12 dpi (Nlrc3^−/− mice, n=25, WT mice, n=20) by real-time PCR. The limit of detection (LOD) was indicated. Bars represented means ± SD. Asterisks indicate a significant difference in the quantity of viral RNA loads (p < 0.05).