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. 2021 Jul 16;12:692509. doi: 10.3389/fimmu.2021.692509

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Copyright © 2021 Ma, Zhang, Shu, Liu, Sun, Hou, Lv, Ying, Wang, Jin, Liu and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of HTNV nucleoprotein (NP) in spleen and kidney samples obtained from Nlrc3^−/− and WT mice after viral challenge. Samples were collected on 3, 6, 9, and 12 dpi for Western blot and immunohistochemistry (IHC) analysis. (A) Western blot results of the kidneys of Nlrc3^−/− and WT mice. Lanes represent corresponding time points. (B) Densitometric analysis of band intensity for A (Nlrc3^−/− mice, n=15; WT mice, n=10). (C) Western blot results of the spleens. (D) Densitometric analysis of band intensity for C (Nlrc3^−/− mice, n=25; WT mice, n=20). IHC staining of mouse kidney (E) and spleen sections (F) to detect HTNV nucleocapsid protein in Nlrc3^−/− (top) and WT mice (bottom) after infection. HTNV NP (green), and DAPI (blue). IHC images are representative of at least three samples. Scale bars, 50 μm. *p < 0.05; **p < 0.01.