Fig. 1.

Detection of HLA-ABC and HLA-DQ Antigen in Conditioned Media Obtained from Non-Invasive and Invasive Lung Carcinoma Cell Lines. Human adenocarcinoma cell lines H358 and H1703 were cultured in RPMI-1640 + 10% FBS and antibiotics, as defined in Methods. Protein concentration of 10 mL of cell-conditioned media from each cell line was determined via BCA and equal amounts of total protein were loaded on to a SDS-PAGE gel. The proteins were transferred on to nitrocellulose membrane, immunoblotted with antibodies specific to HLA-ABC and HLA-DQ and developed using ECL reagents. Panel A shows a membrane immunoprobed with HLA-ABC antibody, whereas Panel B shows a membrane probed with the HLA-DQ antibody. Lane 1: control medium; Lane 2: conditioned medium from non-invasive H358 cells and Lane 3: conditioned medium from invasive H1703 cells. Panel C shows the densitometry analysis from the QuantityOne software (BioRad), with global background subtraction.