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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: Hum Immunol. 2018 Apr 12;79(7):558–563. doi: 10.1016/j.humimm.2018.04.003

Fig. 2.

Fig. 2.

Box and Whisker Plots for soluble MHC targets. Serum from a total of 50 cases relevant to lung cancer screening were evaluated in this study and consisted of 25 cases of benign lesions and 25 of stage I lung adenocarcinoma (AdCA). Sera was examined using commercial antibodies (as specified in the ‘Methods’ section) for the presence of soluble HLA class I gene products (ABC), which were observed as a 41 kDa (panel A) and 33 kDa (panel B) isoforms. Further, MHC class II molecules were also evaluated, including DQA1, which was observed as both a 28 kDa (panel C) and 33–35 kDa (panel D) isoforms; DQ, observed as 28 kDa (panel E) and 26 kDa (panel F) isoforms; DRB1, as a single 30 kDa isoform (panel G); and DMB, observed also only as a single 29 kDa isoform (Panel H). All values on the ordinate axis are densitometry values presented as a median pixel density (i.e. pixels/mm2) from the western blots analysis performed in Quantity One software package v4.6.6 (BioRad).