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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: J Hepatol. 2021 Feb 18;75(1):163–176. doi: 10.1016/j.jhep.2021.02.004

Figure 5. IL-20 downregulates the expression of IκBζ protein and its target genes in hepatocytes by promoting IκBζ degradation.

Figure 5.

(A, B) Schematic treatment timeline of mouse AML12 hepatocytes. (A) Western blot analysis and quantification of IκBζ expression. (B) RT-qPCR analyses of Nfkbiz and IκBζ-target gene mRNAs at the 3h time point. (C) Serum-starved AML12 cells were treated with IL-20, IL-1β, and/or various inhibitors as indicated. Western blot analysis and quantification of IκBζ expression. (D) Serum-starved AML12 cells were pretreated with IL-20 for 1h, and then stimulated with IL-1β in the presence or absence of MG132 for 2 h. Ubiquitination assay for IκBζ was detected with anti-ubiquitin. Values represent means ± SEM from three to four independent experiments. *P< 0.05, **P< 0.01, ***P< 0.001. K.P.: Klebsiella pneumoniae. IL-20 (50 ng/ml); IL-1β (20 ng/ml); CHX (100 μg/ml); MG132 (20 μM)