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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: J Hepatol. 2021 Feb 18;75(1):163–176. doi: 10.1016/j.jhep.2021.02.004

Figure 6. IL-20 promotes IκBζ degradation in hepatocytes via the induction of NQO1.

Figure 6

(A) Serum-starved AML12 cells or primary hepatocytes were treated with IL-20 (50 ng/ml) for the indicated time points. Western blot analysis and quantification of NQO1 expression. (B) AML12 cells were transfected with control siRNA or Nqo1 siRNA for 24 h, and then treated with IL-1β (20 ng/ml) for the indicated time points. Western blot analysis and quantification of IκBζ expression. (C, D) WT and Il20−/− mice were injected with ConA (12 mg/kg) or K.P. (3000 CFU) for the indicated time points. Liver tissues were subjected to the measurement of Nqo1 mRNA levels (panel C). Representative images of NQO1 (green), hepatocyte marker HNF-4α (red), and nuclei (blue) are shown in panel D. (E-H) C57BL/6N mice were intravenously injected with Ad-Gfp (n=3 in control group, n=6 in ConA-treated group) and Ad-shNqo1 (n=3 in control group, n=6 in ConA-treated group) for 7 days, and then followed by ConA injection. (E) Representative images of NQO1 (red) and nuclei (blue). (F) Western blot analysis and quantification of IκBζ expression. (G) Serum ALT and IL-6 levels. (H) RT-qPCR analyses of hepatic expression of IκBζ-dependent genes. Values represent means ± SEM from three to four independent in vitro experiments or means ± SD from in vivo experiments. *P< 0.05, **P< 0.01, ***P< 0.001.