Fig. 1.

Hrs is dynamically expressed and distributed during myogenesis in C2C12 and primary muscle cells. a Representative Western blotting of myoblast C2C12 cell extracts in Pro status (lane 1) and at 7, 24, 48, 72, and 96 h of differentiation (lanes 2–6) and probed with anti-MHC, -Myogenin as makers of differentiation, and anti-HRS antibody. b Quantification of the Hrs protein level from experiments as presented in a. Data are presented as ratio of Hrs/GAPDH and normalized to the Pro status starting point. Data represent mean +/− SEM. n = 8 experiments. Significance was assessed using a Kruskal–Wallis test; **p < 0.01; ***p < 0.001. c qRT-PCR of Hrs-mRNA from C2C12 cells collected in Pro and at 7, 24, 48, 72, and 96 h of differentiation. Data represent mean +/− SEM, n = 3 experiments. Significance was assessed using a Kruskal–Wallis test; ns not significant. d Representative Western blotting of C2C12 in Pro or at 16 h of differentiation treated with DMSO or 200 nM MG132 proteasome inhibitor. Membrane was probed with anti-HRS, -ubiquitin. GAPDH was used as loading control