Skip to main content
. 2021 Jul 14;7(7):1205–1215. doi: 10.1021/acscentsci.1c00444

Figure 6.

Figure 6

Optical control of Hippo signaling in NIH 3T3 cells with the inhibition of LPA receptor signaling. (A,B) Confocal microscopy images of NIH 3T3 cells treated with AzoLPA (100 nM) or dAzoPA (10 μM) for 1 h with or without 365 nm light and with or without LPA receptor inhibitor (LPAi: Ki16425, 10 μM) and immunostained for YAP. Green, YAP; blue, DAPI (nuclei). Scale bars: 50 μm. (C,D) Quantification of nuclear YAP levels in (A). The plots show mean nuclear YAP intensity in each cell. Black horizontal bars indicate mean (n = 540 (C) and 811 (D)), and vertical error bars indicate standard deviation. Statistical significance was calculated using one-way ANOVA, followed by Tukey’s HSD test.