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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Curr Protoc. 2021 Jan;1(1):e27. doi: 10.1002/cpz1.27

Figure 2. C. neoformans wild-type and mutant strains screened for melanin on Nyjer seed agar.

Figure 2.

Wild type (H99) and putative melanin-defective strains were grown 24 h in YPD media. Strains were washed with PBS and then spotted (5 μl) onto a Nyjer seed agar plate. The plate was incubated for 3 days at 30°C prior to imaging with camera equipped smart phone. The upper left spot is H99 showing well-developed brown pigmentation (melanin). The remaining spots are various mutant strains ranging from no pigmentation (white) to wild type levels of pigmentation.