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. 2021 Jul 29;9(7):e002968. doi: 10.1136/jitc-2021-002968

Figure 1.

Figure 1

CD200 is overexpressed in functional leukemia stem cells. (A) CD200 mRNA expression across immunophenotypically sorted healthy (gray) and leukemic (red) cell populations; mined from de Jonge et al15 [GSE74246] (mean±SD; two-sample t-test). (B) CD200 MFI of paired blast (CD34−) and LSCs (CD34+) (n=28; two-sample t-test). (C) Frequency of CD200+ cells against CD200 MFI in 38 primary patients with AML (red), naive B cells (n=8; green), and CD4+ T cells (n=4; gray). (D) CD200 MFI summary for patients with paired naive B and T cell data (Wilcoxon paired test). (E) Representative gating strategy for FACS sorting CD200+ and CD200− cells from CD34+ AML samples. (F) Number of colonies formed in the CD34+CD200 and CD34+CD200+ fractions (mean±SD; Wilcoxon paired test). (G) CD200 mRNA expression in cell fractions that either did (LSC+) or did not (LSC−) engraft in NSG mice from Ng et al21 [GSE76009] (lines connect cells from the same AML patient; paired t-test). (H) RNA-seq gene expression data for sorted progenitor (GMP) and stem (LSK) cells derived from wild type (WT) and the Tet2–/–;Flt3ITD murine leukemia model [GSE57244].25 (I) Representative gating strategy for CD200+ leukemia (CD45.2) stem cells. (J) Combined leukemic burden for NSG and C57Bl/6 mice from 2 experiments (mean±SD; Wilcoxon). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.