Figure 2.

α-mangostin induces apoptosis in 143B and Saos-2 cells. 143B and Saos-2 cells were treated by different concentrations of α-mangostin (0, 10, 20, 30 μM) for 24 h. (A) Cell apoptosis was detected with Annexin V/PI staining by flow cytometry analysis. (B) The protein levels of cleaved-caspase-3/8 were detected by Western blotting. (C) Z-VAD (20 μM) was applied to the 143B and Saos-2 cells which were treated or untreated by α-mangostin (30 μM), and the cell viability was detected by MTT assay. N = 5, *P < 0.05, **P < 0.01.