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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Mar 16;32(6):1336–1344. doi: 10.1021/jasms.0c00464

Figure 1.

Figure 1.

Evaluation of protein solubility under different TMT labeling conditions. Reduced (reduced by TCEP and alkylated by iodoacetamide) and nonreduced E. coli proteins were mixed with acetonitrile at different ratios. (A) Protein concentrations of the supernatants were measured using BCA assay. (B) The protein content of each supernatant was evaluated using SDS-PAGE.