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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: Anal Chem. 2021 Mar 1;93(10):4479–4487. doi: 10.1021/acs.analchem.0c04624

Figure 1.

Figure 1.

Single-cell MS analysis using the modified spray-capillary. (A) Schematic of the modified spray-capillary device for single-cell microsampling (A. cepa): The laser pulled sample inlet end (1) for penetrating single cells. The extracted cell content was either (2) directly analyzed by MS using an in-house built pressure generator or (3) used to perform online CE-MS analysis in the spray-capillary. The sheathless interface (4) with a porous segment (5) was utilized for ESI-based microsampling and MS coupling. (B) Sample injection flow rates were compared between the original spray-capillary and the modified spray-capillary. The day-to-day performance (C) and batch-to-batch reproducibility (D) were also evaluated. Error bars represent relative standard deviation.