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. Author manuscript; available in PMC: 2021 Jul 30.
Published in final edited form as: J Immunol. 2019 Apr 26;202(11):3187–3197. doi: 10.4049/jimmunol.1900089

FIGURE 1.

FIGURE 1.

Dlg1 is not essential for DC development. (A) Splenic cDCs (CD11c+CD8α+ or CD11c+CD11b+) and CD11c+ BMDCs were purified, and protein expression of Dlg1 was analyzed by Western blot analysis with β-actin as loading control. Data are representative of at least two independent experiments. (B and C) DCs from spleens (B) and lymph nodes (C) were analyzed by flow cytometry for comparison between WT (Cd11c-Cre-GFP) and Dlg1-KO (Dlg1fl/flCd11c-Cre-GFP) mice, respectively. Total cell numbers or percentages of DC subsets were analyzed. Data are representative of at least two independent experiments (four mice in each group). n.s., not significant.