. Author manuscript; available in PMC: 2021 Jul 30.

Published in final edited form as: Chem Res Toxicol. 2018 Nov 28;31(12):1339–1347. doi: 10.1021/acs.chemrestox.8b00211

Table 1.

Carbonyl Delivery for MYO Cigarettes and the 3R4F Research Cigarette with Different Amounts of Charcoal on Both the ISO and HCI Methods^a

brand	cigarette type	charcoal amount mg	formaldehyde μg/puff	acetaldehyde μg/puff	acetone μg/puff	acrolein μg/puff	propionaldehyde μg/puff	crotonaldehyde μg/puff	methyl ethyl ketone μg/puff	puff number
ISO method
Good Stuff Red	MYO	0	2.18 ± 0.11^a	54.14 ± 4.47^a	19.09 ± 0.36^a	2.99 ± 0.15^a	4.91 ± 0.38^a	0.49 ± 0.24^a	2.14 ± 0.14^a	9 ± 1^a
		50	1.83 ± 0.14^b	22.56 ± 6.36^b	5.45 ± 2.23^b	1.69 ± 0.66^b	0.74 ± 0.48^b	0.25 ± 0.08^a	0.18 ± 0.18^b	9 ± 0^a
		50	16%	58%	71%	43%	85%	49%	92%	9 ± 0^a
		200	1.51 ± 0.05^c	5.66 ± 2.41^c	1.54 ± 0.74^c	0.53 ± 0.31^c	0.39 ± 0.03^b	0.18 ± 0.04^a	N.D.^b	9 ± 0^a
		200	31%	90%	92%	82%	92%	63%	~100%	9 ± 0^a
Rave Red	MYO	0	2.22 ± 0.19^a	59.05 ± 4.82^a	18.78 ± 2.66^a	4.90 ± 0.12^a	4.84 ± 0.78^a	0.83 ± 0.17^a	3.30 ± 0.52^a	9 ± 0^a
		50	0.53 ± 0.04^b	22.73 ± 3.96^b	4.46 ± 0.61^b	1.19 ± 0.20^b	1.07 ± 0.12^b	1.29 ± 0.10^b	0.75 ± 0.07^b	9 ± 0^a
		50	76%	62%	76%	76%	78%		77%	9 ± 0^a
		200	0.12±0.15^c	3.81 ± 1.56^c	0.91 ± 0.57^b	0.25 ± 0.13^c	0.43 ± 0.32^b	N.D.^c	0.23 ± 0.11^b	9 ± 0^a
		200	95%	94%	95%	95%	91%	~100%	93%	9 ± 0^a
3R4F	Premade	0	1.96 ± 0.03^a	50.39 ± 2.76^a	17.99 ± 1.05^a	5.44 ± 0.47^a	3.22 ± 0.00^a	1.35 ± 0.02^a	2.59 ± 0.05^a	8 ± 1^a
		50	1.12 ± 0.08^b	24.75 ± 2.25^b	5.87 ± 0.59^b	1.62 ± 0.20^b	0.73 ± 0.13^b	1.29 ± 0.05^a	0.86 ± 0.02^b	8 ± 1^a
		50	43%	51%	67%	70%	77%	4%	67%	8 ± 1^a
		200	0.46 ± 0.11^c	3.52 ± 0.98^c	0.59 ± 0.23^c	0.25 ± 0.18^c	ND^c	ND^b	0.19 ± 0.04^c	8 ± 1^a
		200	77%	93%	97%	95%	~100%	~100%	93%	8 ± 1^a
HCI method
Good Stuff Red	MYO	0	3.80 ± 0.69^a	100.01 ± 9.69^a	41.08 ± 8.36^a	11.60 ± 1.86^a	7.96 ± 1.63^a	2.99 ± 0.72^a	8.20 ± 1.98^a	11 ± 1^a
		50	3.98 ± 1.24^a	64.43 ± 2.27^b	21.39 ± 1.73^b	5.55 ± 0.43^b	4.18 ± 0.07^a,b	2.46 ± 0.20^a	5.27 ± 1.17^a,b	11 ± 0^a
		50		36%	48%	52%	47%	18%	38%	11 ± 0^a
		200	2.67 ± 0.53^a	28.09 ± 8.03^c	7.76 ± 3.14^b	2.33 ± 0.88^b	2.05 ± 1.08^b	0.35 ± 0.13^b	1.79 ± 0.63^b	11 ± 0^a
		200	30%	72%	81%	80%	74%	88%	78%	11 ± 0^a
Rave Red	MYO	0	4.61 ± 1.16^a	115.70 ±2.10^a	46.24 ± 4.85^a	12.92 ± 1.03^a	8.86 ± 0.84^a	4.80 ± 1.07^a	9.62 ± 1.45^a	10 ± 1^a
		50	2.51 ± 0.42^b	70.02 ± 3.45^b	19.10 ± 2.02^b	5.04 ± 0.58^b	4.29 ± 0.51^b	1.29 ± 0.19^b	3.24 ± 0.57^b	10 ± 1^a
		50	46%	39%	59%	61%	52%	73%	66%	10 ± 1^a
		200	1.81 ± 0.71^b	25.45 ± 14.34^c	4.99 ± 3.21^c	1.41 ± 0.98^c	1.16 ± 0.62^c	1.06 ± 0.21^b	0.82 ± 0.37^c	10 ± 1^a
		200	61%	78%	89%	89%	87%	78%	91%	10 ± 1^a
3R4F	Premade	0	5.39 ± 0.18^a	131.05 ± 11.82^a	50.63 ± 5.16^a	15.62 ± 1.19^a	8.88 ± 0.49^a	4.22 ± 0.08^a	10.53 ± 3.38^a	11 ± 0^a
		50	2.77 ± 0.44^b	86.59 ± 8.23^b	25.36 ± 2.05^b	6.19 ± 0.23^b	4.68 ± 0.32^b	1.22 ± 0.42^b	4.56 ± 0.35^b	11 ± 1^a
		50	49%	34%	50%	60%	47%	71%	57%	11 ± 1^a
		200	1.85 ± 0.15^c	31.11 ± 12.43^c	7.86 ± 3.83^c	1.74 ± 0.98^c	1.53 ± 0.68^c	0.47 ± 0.22^b	1.68 ± 0.66^b	10 ± 1^a
		200	66%	76%	84%	89%	83%	89%	84%	10 ± 1^a

Values are mean ± standard deviation with percent reduction relative to 0 mg charcoal. Different superscripts (a, b, or c) within a carbonyl group across charcoal amounts denote significant differences as determined by ANOVA (p < 0.05).